Applying Staining Techniques To View And Identify
Bacteria Essay, Research Paper
Abstraction
The chief aim of this lab was to place different bacteriums by simple, negative, and gm staining. To see each bacterium cell, the bacterium was transferred aseptically to a slide, and they were so viewed by utilizing oil submergence, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive.
Introduction
The intent of this lab was to see the different features of bacteriums by using assorted staining techniques. It is of import to cognize the brand up if a certain bacteriums so an antibiotic may be engineered to destruct the bacteriums. From the gm discoloration, it was possible to find which bacterium was gram positive or gram negative. This is of import because Gram-negative bacteriums are by and large more toxic ( due to the lipopolysaccaride ) are immune to antibiotics than the Gram-positive bacteriums.
Methods
The stuffs used for this lab were:
1. A light microscope
2. Four glass slides
3. And inoculating cringle
4. A Bunsen Burner
5. Bacteria ( E.coli, S. megaterium, B. subtilis, and S. marcesens )
6. Alcohol
7. Staining bowl
8. Methylene blue, oil, nigrosin, crystal violet, I, saffranine
9. Distilled H2O
Three different staining processs were so used for all four types of bacterium. The waies for each staining procedure can be found on pages 18-19 of the lab manual. For simple discoloration, bacterium was removed with a unfertile vaccinating cringle and placed on a glass slide. Methylene blue was so applied until the slide was covered. Distilled H2O was so poured on the slide until the methylene blue was removed. The slide was allowed to dry. Following, oil was placed on the slide so that the oil nonsubjective lens on the light microscope was employed. The bacteria was viewed and a study was made. For the negative discoloration, a loopful of bacterium was placed on a slide. A bead of Nigrosin solution was placed following to the bacteriums, and the blood smearing technique learned in the first lab was applied to cover the slide with nigrosin solution. A study was so made. Gram staining was more complicated. First, two separate types of bacteriums were used. One type was placed on one slide, and the other bacteriums were placed on the opposite side. Both bacteriums were besides placed in the center of the slide so they could be viewed together. Crystal violet, I and saffranine were all placed on the slide and washed off with distilled H2O. Once dried, oil was applied to the slide, and it was ready to be viewed. Again a study was made.
Consequences
The studies were drawn on a separate piece of paper and so revised. A tabular array is
provided.
E.coli B. subtilis S. marcesans S. megaturiun
Size Small, bacillar Small, bacillar Round, really little Round, little bunchs
Elevation Convex Flat Convex Convex
Surface Shiny Rough Red, smooth Smooth
Color White, glistening Pale xanthous Reddish-orange Light purple
Edge Wrinkled Round Round Round
Discussion
The basic intent of this lab exercising was to see the different features of certain types of bacterium. Using the oil submergence lens accomplished this undertaking. The importance of the lens is that it can acquire so near to the slide ( it is fundamentally touching it ) and gives the experimenter a great position of the coveted object. The different staining techniques were really utile. The simple discoloration merely stains the cells, so the basic form of the cell is viewed. With the negative discoloration, the background is stained, so the lift, colour, and surface are more easy observed. The gm discoloration, dyed the bacterium either ruddy or violet by utilizing the I, methylene blue, crystal violet, and saffranine, helped find the difference between the gm negative or Gram-positive bacteriums. If the gm discoloration is negative it will hold a lipopolysaccharide cell wall, if positive, it is a peptidoglycan cell wall. Once this is known, scientists can engineer antibiotics that disrupt the bacterium & # 8217 ; s procedure of protein synthesis. Some different illustrations of this are erythromycin and Achromycin.
Mentions
Appendix- Gene Transfer in the Environment.
Cambell, Neil. Biology. 4th Edition. Benjamin/Cummings Pub. Co. 1996
& lt ;< br />
Abstraction
The chief aim of this lab was to place different bacteriums by simple, negative, and gm staining. To see each bacterium cell, the bacterium was transferred aseptically to a slide, and they were so viewed by utilizing oil submergence, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive.
Introduction
The intent of this lab was to see the different features of bacteriums by using assorted staining techniques. It is of import to cognize the brand up if a certain bacteriums so an antibiotic may be engineered to destruct the bacteriums. From the gm discoloration, it was possible to find which bacterium was gram positive or gram negative. This is of import because Gram-negative bacteriums are by and large more toxic ( due to the lipopolysaccaride ) are immune to antibiotics than the Gram-positive bacteriums.
Methods
The stuffs used for this lab were:
1. A light microscope
2. Four glass slides
3. And inoculating cringle
4. A Bunsen Burner
5. Bacteria ( E.coli, S. megaterium, B. subtilis, and S. marcesens )
6. Alcohol
7. Staining bowl
8. Methylene blue, oil, nigrosin, crystal violet, I, saffranine
9. Distilled H2O
Three different staining processs were so used for all four types of bacterium. The waies for each staining procedure can be found on pages 18-19 of the lab manual. For simple discoloration, bacterium was removed with a unfertile vaccinating cringle and placed on a glass slide. Methylene blue was so applied until the slide was covered. Distilled H2O was so poured on the slide until the methylene blue was removed. The slide was allowed to dry. Following, oil was placed on the slide so that the oil nonsubjective lens on the light microscope was employed. The bacteria was viewed and a study was made. For the negative discoloration, a loopful of bacterium was placed on a slide. A bead of Nigrosin solution was placed following to the bacteriums, and the blood smearing technique learned in the first lab was applied to cover the slide with nigrosin solution. A study was so made. Gram staining was more complicated. First, two separate types of bacteriums were used. One type was placed on one slide, and the other bacteriums were placed on the opposite side. Both bacteriums were besides placed in the center of the slide so they could be viewed together. Crystal violet, I and saffranine were all placed on the slide and washed off with distilled H2O. Once dried, oil was applied to the slide, and it was ready to be viewed. Again a study was made.
Consequences
The studies were drawn on a separate piece of paper and so revised. A tabular array is
provided.
E.coli B. subtilis S. marcesans S. megaturiun
Size Small, bacillar Small, bacillar Round, really little Round, little bunchs
Elevation Convex Flat Convex Convex
Surface Shiny Rough Red, smooth Smooth
Color White, glistening Pale xanthous Reddish-orange Light purple
Edge Wrinkled Round Round Round
Discussion
The basic intent of this lab exercising was to see the different features of certain types of bacterium. Using the oil submergence lens accomplished this undertaking. The importance of the lens is that it can acquire so near to the slide ( it is fundamentally touching it ) and gives the experimenter a great position of the coveted object. The different staining techniques were really utile. The simple discoloration merely stains the cells, so the basic form of the cell is viewed. With the negative discoloration, the background is stained, so the lift, colour, and surface are more easy observed. The gm discoloration, dyed the bacterium either ruddy or violet by utilizing the I, methylene blue, crystal violet, and saffranine, helped find the difference between the gm negative or Gram-positive bacteriums. If the gm discoloration is negative it will hold a lipopolysaccharide cell wall, if positive, it is a peptidoglycan cell wall. Once this is known, scientists can engineer antibiotics that disrupt the bacterium & # 8217 ; s procedure of protein synthesis. Some different illustrations of this are erythromycin and Achromycin.
Bibliography
Mentions
Appendix- Gene Transfer in the Environment.
Cambell, Neil. Biology. 4th Edition. Benjamin/Cummings Pub. Co. 1996
37b
