Isolated Analgesic Drug Essay Sample

a. Lab experiment rubric and informations:
Isolation of the active ingredient in an Analgesic Drug
February 12th. 2013
b. Abstract ( intent ) :
Identify analgetic drugs after insulating the active ingredients from tablets. c. Physical informations tabular array
Drug| Melting point| Brand names|
Acetylsalicylic acid| 135-136 °C| Aspirin. ASA. acetyl salicylic acid. generic acetylsalicylic acid. aspirin. | Acetaminophen| 169-170. 5 °C| Tylenol. Datril. Panadol. nonaspirin hurting stand-in. | Ibuprofen| 75-77 °C| Advil. Brufen. Motrin. Nuprin. |

d. Procedure:
Extraction of active ingredient:
Two tablets Ibuprofen ( 0. 400g ) was crushed into pulverization between 2 pieces of weighing paper by a stamp. Ibuprofen contained a ruddy coat. was odourless. unit of ammunition and long as a regular tablet. After first crush. forceps were used to take ruddy fragments. The pulverization after oppressing had white colour and besides odorless. All white pulverization was added to a 3mL conelike phial. Next. 2mL of Methanol was added to the phial by a graduated Pasteur pipet. Then the phial was capped and mixed by agitating. During the experiment. the cap was loosened one time to let go of any force per unit area that may hold built up in the phial. A nebulose suspension appeared at underside of the phial after agitating about 4-5 proceedingss. Until the precipitate had settled wholly. the liquid stage was transferred to a extractor tubing and this extraction was repeated with a 2nd 2mL of Methanol. The liquid was clearer after 2nd shaking. After that. the mixture was centrifuged about 2 proceedingss and 30 seconds ( puting high velocity at 2 ) . After extractor. clear liquid appeared above the white-pink solid. Following. the liquid was transferred to a regular trial tubing. Column Chromatography:

A Pasteur pipet was used to fix an alumina column. The little ball cotton was pushed down to the underside of pipet by a wooden-stick. Alumina ( 0. 5g ) was added to the pipet ( about about 2cm high of aluminum oxide in the pipet ) . A little beaker was placed under the column. To the column. 2mL of methyl alcohol was added and the liquid was allowed to run out until the Methanol reached the top of the aluminum oxide. The solution incorporating the drug from trial tubing was so transferred to the column. After that. an extra 1ml of methyl alcohol was added to the column. The filtrate was clear and odorless. The filtrate was placed into a little beaker. covered with a ticker glass ; the dissolver was evaporated overnight for a couple yearss. Vaporization of dissolver:

After wholly dry. the merchandise had appeared as a white crystal signifier at the underside of the beaker and had no odor. Methanol was heated to a boiling point at 65 °C. Next. methyl alcohol was poured into the crystal and stirred. The solution was left to chill to room temperature on a battalion of towels on top of the tabular array. When cooled. the solution was placed in an ice bath for 10-15 proceedingss. Vacuum Filtration:

A Hirsch funnel was foremost set up. The filter paper was moistened by a few beads of methyl alcohol. The stuff was transferred to the Hirsch funnel by a micro spatula. The crystal was allowed to dry about 5-7minutes. and so the compound looked somewhat gluey. The dried crystals were scraped from the filter paper onto the ticker glass. Next. the ticker glass with the crystal was weighed to cipher the weight of analgetic drug. The crystals or merchandises were crushed once more into pulverization to find their thaw point. e. Data presentation

Items| Measurements|
Mass of beaker + crude| 20. 401g|
Mass of ticker glass| 25. 431g|
Mass of ticker glass + pure| 25. 600g|
M. P of concluding product| 77-80 °C|
Literature M. P of Ibuprofen| 75-77 °C|

Mass of concluding product= ( mass of ticker glass + pure ) – ( mass of ticker glass ) =25. 600g – 25. 431g
= 0. 169g
Weight of substance isolated
Weight per centum recovery= x100 %
Weight of original stuff
0. 169g
= x100 %
0. 400g
=42. 3 %

f. Data Analysis:
*The concluding merchandise was a white crystal. The mass of cured merchandise was 0. 169g and the per centum recovery of this merchandise was 42. 3 % which is about 57. 7 % lower than 100 % . *The runing point of concluding merchandise was recorded at 77-80 °C which is about 2-3 grades higher than the scope ( 75-77 °C ) in the research lab text edition. g. Decision

In this experiment. the mass of cured merchandise was 0. 169g and the per centum recovery of this merchandise was 42. 3 % which is about 57. 7 % lower than 100 % . This consequence was comparatively low. but several factors during the experiment may hold affected the recovery loss. First. when Ibuprofen was crushed. the white pulverization leaked out a small. The merchandise in this experiment had to be transferred into another container like reassigning the merchandise from the conelike phial to the extractor tubing. or from centrifuge tubing to a little beaker. The drug was left behind and it could hold besides dropped into the beaker when during filtration with the cotton ball. The more stairss of reassigning the merchandise meant the more sum of the merchandise lost. The runing point of concluding merchandise was recorded at 77-80 °C which is about 2-3 grades higher than the scope ( 75-77 °C ) . Therefore. the merchandise was contaminated. The ground for that is the merchandise may still hold contained H2O. Water could hold come from the air or environing country during the drying procedure. Post- Lab Questions: 1. 4. 5. 6.

1. The per centum recovery was less than 100 % because the drug leaked during the experiment particularly when Methanol was placed into the Pasteur pipet. The drug leaked when it was crushed or transferred. and it was left behind after extractor. Not adequate Methanols may hold been added. 4. The alumina column was used for sublimating an analgetic drug. 5. Percentage recovery

Weight of substance isolated
Weight per centum recovery= x100 %
Weight of original stuff
0. 185g
= x100 % = 52. 9 %
0. 350g
6. The runing point was low and the thaw scope of the merchandise was so broad because the merchandise was still contaminated ( it contained some excess merchandise that isn’t Acetaminophen which blocked its runing point to make the original thaw point ) . It besides contained H2O that could hold lead to incorrect runing points of the concluding merchandise.