Molecular Biology Essay Sample
Before finishing this assignment. read the Molecular Biology I and II Lab Exercise posted on CourseWeb. You should non re-type the inquiries. but the replies must be typed and written in complete sentences. You will turn in a difficult transcript to your teacher and subject an electronic transcript to Turnitin. com utilizing the registration watchword pittbio2013 and the category ID provided by your teacher. Directions for subjecting an assignment to Turnitin. com are posted on CourseWeb.
This assignment must be completed independently. NO GROUP WORK. Failure to follow this way will ensue in punishment for misdemeanor of academic unity.
1. What is a plasmid? How is it different from genomic or chromosomal Deoxyribonucleic acid?
2. What does the term “transformation” mean as it applies to molecular biological science? Are YOU transforming the E. coli?
3. E. coli cells that contain pAMP will be able to turn on which of the undermentioned LB-agar home bases – LB-KAN. LB-AMP. and/or LB-AMP/ARA? Explain how the plasmid permits E. coli cells to turn on the home base ( s ) .
4. E. coli cells that contain pKAN will be able to turn on which of the undermentioned LB-agar home bases – LB-KAN. LB-AMP. and/or LB-AMP/ARA? Explain how the plasmid permits E. coli cells to turn on the home base ( s ) .
5. E. coli cells that contain pGLO will be able to turn on which of the undermentioned LB-agar home bases – LB-KAN. LB-AMP. and/or LB-AMP/ARA? On which home base ( s ) will cells low in the presence of UV visible radiation? Explain how the plasmid permits E. coli cells to glow and/or grow on the home base ( s ) .
6. Restriction enzymes cut each plasmid into fragments of known base brace ( bp ) lengths. These are the “expected” base brace lengths. Use the plasmid maps on page 3 of the lab exercising ( Figure 1 ) to find the “expected” base brace lengths of pAMP. pKAN. and pGLO Deoxyribonucleic acid after digestion with BamH I and Hind III.
7. Imagine that you digested pKAN. pAMP. and pGLO Deoxyribonucleic acid with BamH I and Hind III. Following digestion. you loaded the cut plasmid into an cataphoresis gel. You loaded the Lambda criterion in the first good. digested pKAN Deoxyribonucleic acid in the 2nd well. pAMP in good 3. and pGLO in good 4. What would the gel expression like after cataphoresis and DNA staining? a. Pull a image demoing all Deoxyribonucleic acid fragments.
b. Label the length of each DNA fragment in base brace. c. Be certain to pull the Wellss to bespeak the gel’s directivity. d. Your image can be hand-drawn on your difficult transcript and turned into your teacher. It is non required on the electronic transcript that will be submitted to TurnItIn. com.
8. What is the intent of the Lambda DNA criterion?
9. Explain how limitation enzymes and electrophoresis let us to find the genotype of E. coli.
10. How does the mini-prep protocol separate plasmid Deoxyribonucleic acid from chromosomal DNA? Describe any relevant solutions and processs.
11. How does the mini-prep process separate plasmid Deoxyribonucleic acid from lipoids and proteins? Describe any relevant solutions and processs.
